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The sterilization of honey with cobalt 60 gamma radiation: a study of honey spiked with spores of Clostridium botulinium and Bacilus subtilus

Experientia, Basel, 1995; 986-989. Postmes T, Boogaard vd A, Hazen M, Department of Internal Medicine and Department of Medical Microbiology, Academic Hospital Maastricht (NL).

Unprocessed honey is a recognized wound healing remedy. However, to make clinical use of honey acceptable,
it should be sterile. To find the lowest dose of irradiation needed for sterilization, six batches of honey (a-f ) were gamma irradiated. with 6, 12, 18, 22 and 25 kGy Cobalt-60. All batches spiked with approximately 10 (6) spores from Cl.Botulinum or B. subtilis proved to be sterile after irradiation with a dose of 25 kGy.

[ We are now aware that this article did not study the effects of the irradiation on the properties of the honey and that ointments made with irradiated honey are rapidly ageing and produce gas which leads to and explosive waste of the ointment when the tubes are opened.]

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